The Best HPLC
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liquid chromatography (HPLC)
High-performance liquid chromatography (HPLC) is a form of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by injecting a plug of the sample mixture onto the column. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Solvents must be degassed to eliminate formation of bubbles. The pumps provide a steady high pressure with no pulsating, and can be programmed to vary the composition of the solvent during the course of the separation. Typical detectors rely on a change in refractive index, UV-VIS absorption, or fluorescence after excitation with a suitable wavelength.
What constitutes a basic HPLC system?
An HPLC system consists of the following instruments:
These instruments are typically small enough to be placed on bench tops in laboratories. Although they are often purchased as separate instruments, some manufacturers combine them into self-contained systems in a single cabinet.
Who uses HPLC techniques?
The following is just a brief list of the types of companies and institutions that make use of HPLC instruments in their work: